ms α g3bp2 Search Results


ms α g3bp2  (Proteintech)
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Proteintech ms α g3bp2
(A) Endogenous ZCCHC3 protein enters SGs marked by endogenous G3BP Stress Granule Assembly Factor 2 <t>(G3BP2)</t> in stressed HEK 293T cells. (B) Summarizing the data of micrograph experiments (C-E), bar graphs were generated by counting at least 200 cells for each transfection. n = number of experimental replicates. (C) FLAG-tagged ZCCHC3 does not form SGs in unstressed U2OS-WT cells, (D) but does when the cells are stressed with Na-arsenite. (E) However, ZCCHC3-FL-containing granules do not form in G3BP1/2 U2OS-DKO cells, even when stressed with Na-arsenite. (F) Similarly, endogenous ZCCHC3 detected by α-ZCCHC3-Av antibody does not form granules in stressed U2OS-DKO cells. NT: no treatment. Cell nuclei were stained with Hoechst 33342 (right-most panels). Size bars are 10 μm.
Ms α G3bp2, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ms+%CE%B1+g3bp2/G3BP2+Antibody/pmc10351740-229-50-71
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ms α g3bp2 - by Bioz Stars, 2026-09
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(A) Endogenous ZCCHC3 protein enters SGs marked by endogenous G3BP Stress Granule Assembly Factor 2 (G3BP2) in stressed HEK 293T cells. (B) Summarizing the data of micrograph experiments (C-E), bar graphs were generated by counting at least 200 cells for each transfection. n = number of experimental replicates. (C) FLAG-tagged ZCCHC3 does not form SGs in unstressed U2OS-WT cells, (D) but does when the cells are stressed with Na-arsenite. (E) However, ZCCHC3-FL-containing granules do not form in G3BP1/2 U2OS-DKO cells, even when stressed with Na-arsenite. (F) Similarly, endogenous ZCCHC3 detected by α-ZCCHC3-Av antibody does not form granules in stressed U2OS-DKO cells. NT: no treatment. Cell nuclei were stained with Hoechst 33342 (right-most panels). Size bars are 10 μm.

Journal: PLOS Genetics

Article Title: ZCCHC3 is a stress granule zinc knuckle protein that strongly suppresses LINE-1 retrotransposition

doi: 10.1371/journal.pgen.1010795

Figure Lengend Snippet: (A) Endogenous ZCCHC3 protein enters SGs marked by endogenous G3BP Stress Granule Assembly Factor 2 (G3BP2) in stressed HEK 293T cells. (B) Summarizing the data of micrograph experiments (C-E), bar graphs were generated by counting at least 200 cells for each transfection. n = number of experimental replicates. (C) FLAG-tagged ZCCHC3 does not form SGs in unstressed U2OS-WT cells, (D) but does when the cells are stressed with Na-arsenite. (E) However, ZCCHC3-FL-containing granules do not form in G3BP1/2 U2OS-DKO cells, even when stressed with Na-arsenite. (F) Similarly, endogenous ZCCHC3 detected by α-ZCCHC3-Av antibody does not form granules in stressed U2OS-DKO cells. NT: no treatment. Cell nuclei were stained with Hoechst 33342 (right-most panels). Size bars are 10 μm.

Article Snippet: Commercial antibodies included rabbit (rb) α-SKIV2L2 (MTR4) (A13258), rb α-ZCCHC3 (A17235; α-ZCCHC3-Ab) (Abclonal), rb α-ZCCHC3 (ARP50730_P050; α-ZCCHC3-Av) (Aviva Systems Biology), mouse (ms) α-DYKDDDDK (binds FLAG-tag, 9A3), rb α-DYKDDDDK (D6W5B), ms α-HA-tag (6E2), rb α-HSP90 (#4874), and rb α-ZCCHC3 (#65321; α-ZCCHC3-CS) (Cell Signaling Technology), ms α-FLAG-M2 (F1804/F3165) (MilliporeSigma), ms α-DIS3 (PCRP-DIS3-1A7), ms α-G3BP2 (PCRP-G3BP2-1C7) (Deveopmental Studies Hybridoma Bank), rb α-EXOSC3 (15062-1-AP), rb α-MOV10 (10370-1-AP), rb α-alpha tubulin (11224-1-AP), rb α-ZC3HAV1 (16820-1-AP, α-ZAP) (ProteinTech), ms α-V5-tag (Thermo Fisher Scientific), goat (gt) α-eIF3η (N-20), and gt α-TIA1 (C-20) (Santa Cruz Biotechnology).

Techniques: Generated, Transfection, Staining